李树杰 教授

1. 基于物理学技术（包括荧光淬灭、荧光共振能量转移FRET、核磁共振NMR和结晶生长等技术）抗新型冠状病毒 SARS-CoV-2感染的软凝聚态纳米凝胶的研发及作用机制研究。

2. 基于物理学技术，高级美白、亮肤、祛斑、祛痘、祛皱和抗炎软凝聚态纳米精华液和面膜的研发及作用机制研究。

3. 基于物理学技术，口臭、牙周炎治疗的纳米凝胶的研发及作用机制研究。

4. 生物大分子（蛋白质、生物多聚物）的提取及工业化应用。

      本研究室长期以来从事上述研究工作，积累了丰富的经验。建立了南开大学连云港大健康研发中心（面积700平，附有3套配有空调、热水器等设备齐全的公寓）产学研平台。

      建立了和日本京都大学原子炉实验所森本幸生教授，瑞士日内瓦大学Nicolas Demaurex教授，南丹麦大学Qihua Tan教授，美国加利福尼亚大学Francesco Tombola教授实质性的合作研究和研究生的联合培养。

       欢迎物理学院的同学加入我们团队。

李树杰教授十几年来一直潜心从事质子通道Hv1的研究工作，取得了国际同行认可的研究成果。解析了Hv1胞内C端结构域的晶体结构，确定了该结构域不但是整体蛋白形成二聚体的必要结构域，并且对该蛋白在细胞膜上的定位起决定性作用(Li et al., J. Biol. Chem. 285, 12047-12054 (2010)) ；在国际上首先发现了Hv1在高转移癌组织和细胞中高表达，具有调节肿瘤细胞内外pH值的功能，对肿瘤的酸性微环境的形成起重要作用(Wang et al.,J. Biol. Chem.287, 13877-13888 (2012)) .

在国际上首次发现了Hv1在人、小鼠和大鼠的胰岛β细胞中大量表达。用siRNA下调Hv1的表达显著抑制了提取的小鼠胰岛和INS-1（832/13）β细胞在葡萄糖或K⁺刺激的胰岛素分泌，同时抑制了Ca²⁺内流(Zhao et al., BBRC. 468, 746-751 (2015)) 。进一步的研究发现敲除Hv1明显抑制了促泌剂刺激的胰岛素分泌；敲除Hv1对胰岛素的合成和成熟有一定程度的影响；通过对Hv1基因敲除鼠的体内实验发现了Hv1基因敲除鼠空腹血糖偏高，明显的糖耐受异常，糖刺激的胰岛素分泌缺失；敲除Hv1抑制了葡萄糖刺激的膜去极化和Ca²⁺内流。

[1] Li J, Zhang Y, Pang H, Li Shu Jie#.Heparin interacts with the main protease of SARS-CoV-2 and inhibits its activity.Spectrochim Acta A Mol Biomol Spectrosc. 2021 Nov 15;267:120595.（#Corresponding author）

[2] Du H, Li Shu Jie#. Inhibition of porphyra polysaccharide on xanthine oxidase activity and its inhibition mechanism. Spectrochim Acta A Mol Biomol Spectrosc. 2021 Sep 28;266:120446.(#Corresponding author)

[3] Pang H, Li Shu Jie#, Wang Y, Su X, Gao Y, Li SJ. Mice lacking the proton channel Hv1 exhibit sex-specific differences in glucose homeostasis. J Biol Chem. 2021 Sep 20;297(4):101212.(#Corresponding author)

[4] Pang H, Li J, Miao Z, Li Shu Jie#. Inhibitory effects of chondroitin sulfate on alpha-amylase activity: A potential hypoglycemic agent. Int J Biol Macromol. 2021 Aug 1;184:289-296.(#Corresponding author)

[5] Du H, Pang H, Gao Y, Zhou Y, Li Shu Jie#. Deficiency of voltage-gated proton channel Hv1 aggravates ovalbumin-induced allergic lung asthma in mice. Int Immunopharmacol. 2021 Jul;96:107640.(#Corresponding author)

[6] Pang H, Wang X, Zhao S, Xi W, Lv J, Qin J, Zhao Q, Che Y, Chen L, Li Shu Jie#. Loss of the voltage-gated proton channel Hv1 decreases insulin secretion and leads to hyperglycemia and glucose intolerance in mice.J Biol Chem. 2020 Mar 13;295(11):3601-3613.(#Corresponding author)

[7] Pang H, Li J, Du H, Gao Y, Lv J, Liu Y, Li Shu Jie#. Loss of voltage-gated proton channel Hv1 leads to diet-induced obesity in mice. BMJ Open Diabetes Res Care. 2020 Feb;8(1). pii: e000951.(#Corresponding author)

[8] Wang X, Gao YT, Jiang D, Wang Y, Du H, Lv J, Li Shu Jie#. Hv1-deficiency protects β cells from glucotoxicity through regulation of NOX4 level.Biochem Biophys Res Commun. 2019 May 28;513(2):434-438.(#Corresponding author)

[9]Feng W, Li J, Liang Y, Zhang Y, Li Shu Jie#.Structural characterisation of a full-length mitochondrial outer membrane TOM40 preprotein translocase: implications for its interaction with presequence peptides.Eur Biophys J. 2019 Jan;48(1):35-43.(#Corresponding author)

[10]Wang X, Xi W, Qin J, Lv J, Wang Y, Zhang T, Li Shu Jie#.Deficiency of voltage-gated proton channel Hv1 attenuates streptozotocin-induced β-cell damage.Biochem Biophys Res Commun. 2018 Apr 15;498(4):975-980.(#Corresponding author)

[11]Zhao Q, Zuo W, Zhang S, Zhang Y, Li C, Li Shu Jie#.Proton pump inhibitors have pH-dependent effects on the thermostability of the carboxyl-terminal domain of voltage-gated proton channel Hv1.Eur Biophys J. 2018 Apr;47(3):237-247. (#Corresponding author)

[12] Feng W, Zhang Y, Deng H, Li Shu Jie#. Interaction of divalent metal ions with human translocase of inner membrane of mitochondria Tim23. Biochem. Biophys. Res. Commun. 475, 76-80 (2016) (#Corresponding author)

[13] Zhao Q, Che Y, Li Q, Zhang S, Gao YT, Wang Y, Wang X, Xi W, Zuo W, Shu Jie Li#. The voltage-gated proton channel Hv1 is expressed inpancreatic islet β-cells and regulates insulin secretion. Biochem. Biophys. Res. Commun. 468, 746-751 (2015) (#Corresponding author)

[14] Zhao Q, Li C, Shu Jie Li#. The pH-sensitive structure of the C-terminal domain of voltage-gated proton channel and the thermodynamic characteristics of Zn²⁺ binding to this domain. Biochem. Biophys. Res. Commun. 456, 207-212 (2015) (#Corresponding author)

[15] Zhao Q, Zhang Y, Shu Jie Li#. Interaction of divalent metal ions with the carboxyl-terminal domain of human voltage-gated proton channel Hv1.Biometals 27, 793-802 (2014) (#Corresponding author)

[16] Zhang S, Wang Y, Shu Jie Li#. Lansoprazole induces apoptosis of breast cancer cells through inhibition of intracellular proton extrusion. Biochem. Biophys. Res. Commun. 13, 448, 424-429 (2014) (#Corresponding author)

[17] Wang Y, Wu X, Li Q, Zhang S, Shu Jie Li#. Human voltage-gated proton channel Hv1: a new potential biomarker for diagnosis and prognosis of colorectal cancer. PLoS One, 8, e70550 (2013). (#Corresponding author)

[18] Wang Y, Zhang S, Shu Jie Li#. Zn2+ induces apoptosis in human highly metastatic SHG-44 glioma cells, through inhibiting activity of the voltage-gated proton channel Hv1. Biochem. Biophys. Res. Commun. 438, 312-317 (2013) (#Corresponding author)

[19] Zhang Y, Deng H, Zhao Q, Shu Jie Li#. Interaction of presequence peptides with human translocase of inner membrane of mitochondria Tim23. Biochem. Biophys. Res. Commun. 437, 292-299. (2013) (#Corresponding author)

[20] Shu Jie Li#, Wang Y, Wu X, Che Y, Li Q. Clinicopathological and biological significance of human voltage-gated proton channel Hv1 over-expression in breast cancer. J. Biol. Chem. 287, 13877-13888 (2012) (#Corresponding author)

[21] Zhang Y, Deng H, Zhao Q, Shu Jie Li#. Interaction of presequence with human translocase of inner membrane of mitochondria Tim50. J. Phys. Chem. B 116, 2990-2998 (2012) (#Corresponding author)

[22] Zhang Y, Deng H, Zhang X, Shu Jie Li#. Interaction of divalent metal ions with human translocase of inner membrane of mitochondria Tim50. Biochem. Biophys. Res. Commun. 428, 365-370 (2012) (#Corresponding author)

[23] Zhang Y, Xu Y, Zhao Q, Ji Z, Deng H, Shu Jie Li#. The structural characteristics of human preprotein translocase of the inner mitochondrial membrane Tim23: Implications for its physiological activities. Protein Expr. Purif. 82, 255-262 (2012) (#Corresponding author)

[24] Shu Jie Li#, Wang Y, Pan J, Che Y, Yin J, Zhao Q. Specific expression of the human voltage-gated proton channel Hv1 in highly metastatic breast cancer cells, promotes tumor progression and metastasis. Biochem. Biophys. Res. Commun. 412,353-359 (2011) (#Corresponding author)

[25] Zhang Y, Xu Y, Zhao Q, Ji Z, Li Q, Shu Jie Li#. Expression and structural characterization of human translocase of inner membrane of mitochondria Tim50. Protein Expr. Purif. 80, 130-137 (2011) (#Corresponding author)

[26] Shu Jie Li#, Zhao Q, Zhou Q, Unno H, Zhai Y, Sun F. The role and structure of the carboxyl-terminal domain of the human voltage-gated proton channel Hv1. J. Biol. Chem. 285, 12047-12054 (2010) (#Corresponding author)

[27] Shu Jie Li#, Zhao Q, Zhou Q, Zhai Y. Expression, purification, crystallization and preliminary crystallographic study of the carboxyl-terminal domain of the human voltage-gated proton channel Hv1, Acta Crystallogr. 65F,279-281 (2009) (#Corresponding author)

申请六项发明专利：

1. 一种以电压门控质子通道Hv1作为乳腺癌治疗的靶向位点的基因药物（专利号：ZL 201010297433.X)

2. 一种人源乳腺癌抗原及其抗体（申请号：201010297418.5)

3.  一种乳腺癌、结直肠癌和胰腺癌的诊断和预后标志物（申请号：201210070743.7)

4.  一种以电压门控质子通道Hv1为靶点的人乳腺癌治疗药物（申请号：201310249119.8)

5.  氯化锌作为人脑胶质瘤治疗药物的新用途（申请号：201310249008.7)

6. 电压门控质子通道Hv1在治疗糖尿病中的功能和应用（申请号：201611006272.8）